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ENZYMES (INTRODUCTION ) KIT

Enzymes are often present in such small quantities that the amount of enzyme is determined by measuring its ?activity? through the disappearance of a substrate or the appearance of a product. This kit is designed to study the enzyme activity of Invertase, Amylase, and Pepsin using specific enzyme assay systems. It includes five introductory experiments: Demonstrating Invertase Activity, Demonstrating Amylase Activity, Demonstrating Pepsin Activity, Demonstrating the Presence of Amylase in Saliva, and The Effect of Time on the Activity of Invertase. Invertase and amylase activity are measured using DNS color change systems. Visual examination or a spectrophotometer can be used to determine enzyme reaction rates for these systems. Kit includes all necessary chemicals and a reply card for Invertase, Amylase, Pepsin & Hemoglobin to be shipped separately upon request. Reply card items are only shipped within the continental United States. Requires lab glassware; including beakers, test tubes, and graduated cylinders; a hot plate or water bath, and a spectrophotometer or colorimeter, absorption 540 nm (optional). Designed for a class of 24 working in groups of 4. Activities are appropriate for advanced biology classes and recommended for Grades 10 ? 14.

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